Wednesday, March 22
12noon - 1:00pm
290 Hearst Mining Building
Investigating B cell diversity -- Using Nanopore Technology to Improve Single Cell RNAseq.
Assistant Professor of Biomolecular Engineering
UC Santa Cruz
Recent advances in molecular biology protocols and sequencing technology have made it possible to analyze the entire transcriptomes of single cells. Applying this single cell RNAseq technology to B and T cells has the potential to uncover the true diversity of the adaptive immune system. However, Illumina sequencing technology, the current gold standard for RNAseq analysis, is not capable to resolve many of the more complex transcript isoforms present in mammalian transcriptomes. Therefore, we have implemented both library preparation protocols and computational tools to use Oxford Nanopore Technology sequencing for the RNAseq analysis of single cells. This has allowed us to identify high diversity among B cell surface receptor isoforms that Illumina sequencing failed to resolve.