BASF Seminar in Chemical Sciences: Cysteine Arylation to Engineer Peptides and Proteins

Seminar | November 7 | 11 a.m.-12 p.m. | 120 Latimer Hall

 Prof. Bradley Pentelute, Department of Chemistry, MIT

 College of Chemistry

Here we report a robust bioconjugation method using cysteine arylation. This chemistry enables site-specific conjugation at cysteine residues within peptides, proteins, and antibodies. Our two developed approaches use either perfluoroaryl-cysteine SNAr chemistry or organometallic palladium reagents. This work lead to the discovery of a self-labeling four-residue sequence that enables regioselective conjugation at only one cysteine residue within an intact antibody containing natural amino acids. Recently, we discovered a new approach for the native conjugation of complex natural products such as vancomycin onto peptides and proteins without the introduction of linkers or chemical handles.

 Light refreshments will be served at 10:50 at The Coffee Lab, 510-643-0572