High-throughput T cell repertoire profiling enabled systems immunology and immune engineering

Seminar | September 10 | 2-3 p.m. | 177 Stanley Hall

 Ning Jenny Jiang, University of Texas, Austin

 QB3 - California Institute for Quantitative Biosciences

Recent technological advancement have enabled high-throughput measurement of immune receptor sequence repertoire as well as characterizing of T cell phenotype and function at single cell level. However, how to evaluate T cell receptor binding affinity in a high-throughput fashion and decipher the interaction between the ligand repertoire and T cell receptor repertoire are still challenging. In this talk, I will first introduce three different technologies that my lab developed, an ultra-accurate immune repertoire sequencing, a high-throughput TCR affinity measurement, and a high-throughput linking of antigen specificity to T cell receptor sequences. Then, I will give examples on how to use them to study antibody repertoire development and its response to malaria infection in infants and profile T cell repertoire for cancer immunotherapy.