New biological findings which were revealed by imaging studies based on chemical probes

Seminar | January 16 | 11 a.m.-12 p.m. | 120 Latimer Hall

 Prof. Kazuya Kikuchi, Osaka University

 College of Chemistry

One of the great challenges in the post-genome era is to clarify the biological significance of intracellular molecules directly in living cells. If we can visualize a molecule in action, it is possible to acquire biological information, which is unavailable if we deal with cell homogenates. One possible approach is to design and synthesize chemical probes that can convert biological information to chemical output.

Fluorescence protein labeling by synthetic probes is a robust approach to investigate protein function and localization inside living cells. Its potential advantage is that various fluorescent molecules are available as labeling reagents, and the timing of protein labeling is easily controlled. In the reported labeling system, washing procedures are required to remove the free probes from cells. However, if the probes are not completely washed out, the remaining probes cause the reduction of the signal-to-noise ratio. For the solution of this limitation photoactive yellow protein (PYP) tag and mutated β–lactamase (BL) tag will be introduced.

 Light refreshments will be served at 10:50 at The Coffee Lab, 510-643-0572