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ORGANIZER;CN="UC Berkeley Calendar Network":
LOCATION:621 Stanley Hall
DESCRIPTION:Microfluidic integration for automated targeted proteomic assays in prostate cancer and HIV diagnostics\n\nAlex Hughes\, Herr Lab\, UCB\n\nTuesday (April 24th)\, 6PM\, 421 Stanley Hall\n\nAbstract: Immunoblotting techniques\, which couple protein separations with immunological characterization\, are perhaps the most widely used targeted proteomic tools in the life and medical sciences. Despite their analytical power\, conventional benchtop immunoprobing assays consume tremendous time\, labor\, reagents\, and sample resources. I will describe a newly developed integrated approach to immunoblotting that relies upon a light-activatable\, volume-accessible "LAVAgel" protein sieving and capture matrix that unifies rapid size (isoelectric focusing) or weight-based (SDS-PAGE) protein separations and immunoprobing. This rational engineering design strategy advances analytical technology for automated\, scalable scrutiny of protein targets in complex diagnostic fluids as part of a pipeline to realize personalized proteomics in medicine. I will detail two specific applications of the LAVAgel immunoblot - 1) isoform-resolution measurements of the prostate cancer marker PSA in lysate and blood sera\; 2) A mature\, single-channel western blot assay with total assay times of as little as 10 min\, which we have further developed as a rapid confirmatory HIV diagnostic using human sera. \n\nBio: Alex Hughes is a 4th year PhD candidate in the Herr Lab at UC Berkeley\, with expertise in protein size and charge-based microfluidic immunoblotting. He is currently funded by a National Defense Science and Engineering Graduate (NDSEG) Fellowship from the DoD. Alex has undergraduate degrees in chemical engineering and pharmacology from the University of Auckland\, New Zealand. Here\, he gained experience at Fisher & Paykel Healthcare as a product development engineer\, where he developed innovative signal processing strategies for point-of-care positive airway pressure sleep apnea devices.\n\nAbout the Point-of-Care Diagnostics Idea Lab\nThe Point-of-Care Diagnostics (POCDx) Idea Lab was created as a forum for graduate students who are working in the areas of medical diagnostics and global health to discuss topics relevant to their research. Students working in the areas of engineering\, science\, policy\, and healthcare are encouraged to attend. The bi-weekly meetings will be informal and will usually include one or two short presentations by graduate students\, followed by discussion. Students are able to interact with leaders in point of care diagnostics across all relevant disciplines\, and are also encouraged to present their own work for critical feedback from a diverse and motivated group.\nCheck out the new website! http://www.pocdx.org
URL:http://events.berkeley.edu/index.php/calendar/sn/pubaff.html?event_ID=46679&view=preview
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