Dissertation Talk: Chip-Scale Fluorescence Microscope
Seminar: Dissertation Talk: EE | May 9 | 3:30-4:30 p.m. | Cory Hall, 490H (Immersion Room in Swarm Lab)
Efthymios Philip Papageorgiou
This talk presents an image sensor incorporating angle-selective gratings for resolution enhancement in contact imaging applications. Optical structures designed in the CMOS metal layers above each photodiode form the angle-selective gratings that limit the sensor angle of view to 18 degrees, rejecting background light and deblurring the image. The sensor is designed specifically for the intraoperative detection of microscopic residual disease, small clusters of hundreds to thousands of cancer cells left behind after the gross tumor is removed during a surgical resection. A custom 15um thickness amorphous silicon optical wavelength filter is used alongside the image sensor in order to perform fluorescence imaging. The filter is compatible with several clinically tested fluorophores, such as IR700DX.
We demonstrate imaging and detection of foci containing less than 200 cancer cells labeled with fluorescent biomarkers in 50ms. We also demonstrate the detection of microscopic cancer using human tissue and residual tumor in mice models. The absence of large optical elements enables extreme miniaturization, allowing manipulation within a small, morphologically complex, tumor cavity.