Molecular mechanism of DNA replication - from origins to forks and factories

Seminar | December 2 | 4-5 p.m. | 106 Stanley Hall

 Huilin Li, Van Andel Research Institute

 College of Chemistry

In eukaryotes, DNA replication origins are activated in the late G1 phase of cell cycle, in which Origin Recognition Complex recruits the helicase core Mcm2-7 onto DNA and assemble an inactive double-hexamer. The Mcm2-7 double-hexamer is converted to two active CMG (Cdc45-Mcm2-7-GINS) helicases at the G1-to-S transition. And in the S phase, the CMG helicases works with DNA polymerases, primases and numerous additional protein factors to synthesize DNA. In my talk, I will describe molecular insights we have obtained by cryo-EM imaging of several protein complexes involved in the DNA replication process.