DNA Origami Tools for Cryo-EM: Nano Seminar Series

Seminar | May 3 | 2-3 p.m. | 4 LeConte Hall

 Prof. Shawn Douglas, UC San Franciscso, Cellular Molecular Pharmacology

 Berkeley Nanosciences and Nanoengineering Institute

We have created DNA origami nanostructures to overcome challenges in structure determination of certain small ( under 100 kDa ) proteins and nucleic acids by cryo-electron microscopy.

We aimed to use the nanoscale positional control afforded by origami to overcome issues related to particle contrast, preferential orientation, and pseudo-symmetry that can confound the 3D reconstruction process. In the course of this project, we found it necessary to improve our nanostructure design methods to reach the quality of DNA origami required for our application.

We developed new algorithms for fast, automated sequence optimization and 3D structure prediction of DNA origami, and plan to release this software as part of an online toolkit later this year.
Shawn Douglas did his BS in computer science at Yale, PhD in biophysics at Harvard with George Church, and joined UCSF in 2012.