BASF Lecture in Chemical Sciences: Organic chemistry of natural proteins under crude conditions
Seminar | February 28 | 11 a.m.-12 p.m. | Pitzer Auditorium, 120 Latimer Hall
Prof. Itaru Hamachi, Department of Synthetic Chemistry and Biological Chemistry Graduate School of Engineering Kyoto University, Kyoto and CREST/JST, JAPAN
Traditionally, proteins analysis has been conducted under the purified dilute aqueous conditions in most cases. However, it is now being recognized that structure and functions of natural proteins in live systems are rather different from those under such pure conditions. Therefore, it is ideal that proteins can be studied in vivo in more-details for deep understanding of these biomolecules. For such objectives, development of chemical methods to selectively label, image and regulate a target protein under live cell (natural) habitats is now highly desired in the recent chemical biology research. I would like to discuss our recent progress in chemistry-based methods for specific labeling of endogenously expressed proteins driven by coupling of selective molecular recognition and reaction, so-called ligand-directed chemistry (LDchem), under live cell conditions. In LDchem, a reactive and cleavable linker is designed to connect a ligand for selective recognition to a protein-of-interest (POI) with a probe to be tethered to the protein surface. LDchem allows for the protein and site selective labeling driven by the proximity effect in live cells, as well as cell lysates and a pure sample of test tubes. The targets are being now extended to various membrane-bound proteins such as folate receptor, GPCR and neurotransmitter receptors (GluR and GABAR) and intracellular proteins. My group believes that such a new chemistry may facilitate various aspects of fundamental chemical researches, in addition to diagnostic or pharmaceutical applications.
Light refreshments served at The Coffee Lab at 10:50am.